Document 0055
 DOCN  M9470055
 TI    The use of a scanning proton microprobe to observe anti-HIV drugs within
       cells.
 DT    9409
 AU    Cholewa M; Legge GJ; Weigold H; Holan G; Birch CJ; Micro Analytical
       Research Centre (MARC), School of Physics,; University of Melbourne,
       Parkville, Vic., Australia.
 SO    Life Sci. 1994;54(21):1607-12. Unique Identifier : AIDSLINE MED/94254632
 AB    A series of inorganic polyanions (viz. heteropolytungstates) has been
       shown to have antiviral activity but there was no evidence to indicate
       that the drugs reached their site of antiviral (HIV) activity intact. We
       have shown that with a scanning proton microprobe it is possible to
       analyse the metal content of individual cells (PBLs) treated with such a
       polyoxometalate drug and to determine the atomic ratio of the metals
       within the cell. This was found to be near that in the drug. The
       distribution of the metals (tungsten and cobalt) within the cell was
       measured and it was shown that both metals were located in the same
       region within the cell. These findings would suggest that the drug had
       entered the cells intact.
 DE    Antiviral Agents/*ANALYSIS/TOXICITY  Cells, Cultured
       Cobalt/*ANALYSIS/TOXICITY  Human  HIV/*DRUG EFFECTS
       Lymphocytes/*CYTOLOGY  Phosphorus/ANALYSIS  Phosphotungstic
       Acid/*ANALYSIS/TOXICITY  Potassium/*ANALYSIS/TOXICITY  Spectrometry,
       X-Ray Emission/*METHODS  Support, Non-U.S. Gov't  Tungsten/*ANALYSIS
       JOURNAL ARTICLE

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